Not running properly on all 4

[IMM]

Ok i have had the carbs off again taken the float chamber off changed all the jets form a spear set of carbs re-cleaned all the jets in No.1 carb but it all back together and still the same, ok so i did the easy start and it started to run on all 4 whoop whoop  then back the 3 after it burnt off, while the carbs were off i did check and re check all the diaphragms and all look good with no splits in them, checked the float needle by blowing down the fuel delivery pipe while moving the float and it shut the air on and off  so now i am at a loss as what to do next, just to say i have also readjusted the carbs each one is at two turns from there seats, on No1 i did do a full adjustment while the bike was running and that didn't make any difference to its running on 3 cylinders
I am at a loss with this